Certain Vaginal Bacteria May Protect Against HIV Transmission During Sex

alt

Specific types of Lactobacillus bacteria in the vaginal mucus can trap HIV and may help prevent sexual transmission of the virus to women, according to a report in the October 6 edition of the online journal mBio. Conversely, researchers found that another species associated with bacterial vaginosis may increase susceptibility to HIV infection.

Kenetta Nunn from the University of North Carolina at Chapel Hill and colleagues conducted a laboratory study of barrier properties of the cervicovaginal mucus. This mucus can provide a barrier that stops HIV and other sexually transmitted viruses from reaching target cells in the vaginal epithelium, thereby preventing or reducing infections, they noted as background.

By tracking fluorescent HIV-1 pseudovirus particles in cervicovaginal mucus samples from multiple women, they found that neither mucus pH, total lactic acid levels, or Nugent scores (a system for assessing vaginal bacteria in Pap test samples) were significantly correlated with virus trapping. However, HIV was generally trapped in mucus with high concentrations of D-lactic acid and microbiota dominated by Lactobacillus crispatus. In contrast, HIV diffused rapidly through mucus with low concentrations of D-lactic acid dominated by Lactobacillus iners or with substantial amounts of Gardnerella vaginalis (associated with bacterial vaginosis).

"Our results demonstrate that the vaginal microbiota, including specific species of Lactobacillus, can alter the diffusional barrier properties of [cervicovaginal mucus] against HIV and likely other sexually transmitted viruses and that these microbiota-associated changes may account in part for the elevated risks of HIV acquisition linked to bacterial vaginosis or intermediate vaginal microbiota," the study authors concluded.

Below is an edited excerpt from an American Society for Microbiology press release describing the study and its findings in more detail.

Vaginal Microbes Influence Whether Mucus Can Trap HIV Virus

Washington, DC -- October 6, 2015 -- HIV particles are effectively trapped by the cervicovaginal mucus from women who harbor a particular vaginal bacteria species,Lactobacillus crispatus. The findings, published this week in mBio, an online open-access journal of the American Society for Microbiology, could lead to new ways to reduce or block vaginal transmission of HIV and other sexually transmitted infections (STIs).

"Mucosal surfaces, such as the lung, gastrointestinal tract, or female reproductive tract, are where most infections take place," says Sam Lai, assistant professor of pharmacy and engineering at University of North Carolina at Chapel Hill and senior author on the study. "Our bodies secrete over six liters of mucus everyday as a first line of defense."

Cervicovaginal mucus (CVM) can act as a barrier to prevent pathogens from reaching the underlying vaginal wall cells, but the barrier properties vary greatly from woman to woman and even at different times in the same woman. Lai and his collaborators wanted to know what accounts for those differences.

They collected fresh CVM samples from 31 women of reproductive age, measured various properties of the mucus and used high-resolution, time-lapse microscopy to test whether fluorescent HIV pseudovirus particles became trapped in the mucus or diffused freely.

The researchers observed two distinct populations of CVM samples, one that was very good at trapping HIV and one that was not. Trapping of HIV did not correlate to the mucus' pH, total lactic acid, or Nugent score, a rough measure of vaginal 'health' that reflects how many Lactobacillus bacteria are present compared to other microbes.

One difference between the two groups, higher levels of D-lactic acid in the group that trapped HIV, stood out to the researchers because humans cannot make D-lactic acid. The team suspected that different bacteria living within the mucus layer were responsible for differences in D-lactic acid. When they sequenced ribosomal genes to identify the bacteria within each sample, the researchers found that the samples fell again into two groups.

L. crispatus bacteria dominated the CVM that trapped HIV. In contrast, CVM that failed to trap HIV either possessed a different Lactobacillus species, L. iners, or had multiple bacterial species present including Gardnerella vaginalis -- both conditions that are frequently associated with bacterial vaginosis.

"I was really surprised by how slight differences between Lactobacillus species make a very substantial difference in the barrier properties of mucus," says Lai. In the clinical setting, there is a clear link between bacterial vaginosis and having a higher risk of acquiring and transmitting STI’s. Historically, Lai explains, gynecologists considered vaginal microflora to be healthy if it was dominated by any Lactobacillus species. "But our work shows that from a mucus barrier perspective, that is not a good enough distinction," says Lai.

Healthcare workers should be aware that women who harbor L. iners probably have a substantially higher risk of acquiring STIs. And conversely, a L. crispatus dominant microflora may be more protective against HIV and STIs than previously appreciated.

The group also showed that L. crispatus caused CVM to be more 'sticky' against HIV particles -- rather than making a tighter mucus mesh. The barrier function was not unique to HIV particles and would likely trap other enveloped viruses as well.

Lai notes that CVM can be thought of as a "biological condom" which could potentially be reinforced by altering a woman’s vaginal microbiota. "If we could find a way to tilt the battle in favor of L. crispatus in women, then we would be increasing the barrier properties of their CVM, and improve protection against STIs," he says.

10/7/15

Reference

KL Nunn, YY Wang, D Harit, SK Lai et al. Enhanced Trapping of HIV-1 by Human Cervicovaginal Mucus Is Associated with Lactobacillus crispatus-Dominant Microbiota. mBio 6(5):e01084-15. October 6, 2015.

Other Source

American Society for Microbiology. Vaginal Microbes Influence Whether Mucus Can Trap HIV Virus. Press release. October 6, 2015.